2019 SUMS-RAS

Enabling Technologies, Future Directions

Thursday October 10, 2019
Frances C. Arrillaga Alumni Center

The SUMS Research Applications Symposium is a full day of mass spec-related research, networking, and information on the latest groundbreaking technologies and applications.  The 2019 SUMS-RAS Keynote Speaker, Prof. Garry Nolan, is a pioneer, combining multiple technologies into exciting new enabling methodologies.  As exemplified by the keynote, the symposium theme "Enabling Technologies, Future Directions" celebrates technological innovations and explores future potential.  Come and connect with an enthusiastic community of scientists, and participate in sparking new scientific opportunities and directions.

Prof. Garry P. Nolan Dept. of Microbiology & Immunology, Stanford School of Medicine

Keynote: Pathology from the Molecular Scale on Up

High parameter single cell analysis has driven deep understanding of immune processes.  Using a next-generation single-cell “mass cytometry” platform we quantify surface and cytokine or drug responsive indices of kinase target with 45 or more parameter analyses (e.g. 45 antibodies, viability, nucleic acid content, and relative cell size).  Similarly, we have developed two advanced technologies termed MIBI and CODEX that enable deep phenotyping of solid tissue in both fresh frozen and FFPE formats (50 – 100 markers).   Collectively, the systems allows for subcellular analysis from the 70nm resolution scale to whole tissue in 3D. I will present evidence of deep internal order in immune functionality demonstrating that differentiation and immune activities have evolved with a definable “shape”.  Further, specific cellular neighborhoods of immune cells are now definable with unique abilities to affect cellular phenotypes—and these neighborhoods alter in various cancer disease states.   In addition to cancer, these shapes and neighborhoods are altered during immune action and “imprinted” during, and after, pathogen attack, traumatic injury, or auto-immune disease.  Hierarchies of functionally defined trans-cellular modules are observed that can be used for mechanistic and clinical insights in cancer and immune therapies.

PROGRAM