Instruments
Thermo Exploris 240 LC/MS
Acknowledgement: This work utilized the Thermo Exploris 240 LC/MS system (RRID:SCR_022216) that was purchased with funding from Stanford c-ShARP (RRID:SCR_022986) and was supported by Stanford University Mass Spectrometry (RRID:SCR_017801)."
Thermo Exploris 480 nanoLC/MS
Acknowledgement: This work utilized the Thermo Exploris 480 nanoLC/MS system (RRID:SCR_022215) that was purchased with funding from Stanford c-ShARP (RRID:SCR_022986) and was supported by Stanford University Mass Spectrometry (RRID:SCR_017801)."
Thermo Eclipse nanoLC/MS
The Eclipse was acquired in 2021 with federal funding that must be acknowledged: This work utilized the Thermo Orbitrap Eclipse nanoLC/MS system (RRID:SCR_022212) that was purchased with funding from National Institutes of Health Shared Instrumentation grant 1S10OD030473, and was supported by Stanford University Mass Spectrometry (RRID:SCR_017801)."
Thermo QE-HFX nanoLC/MS
The HFX is a fast-scanning quadrupole-Orbitrap, which improves proteomic depth for discovery applications, and integrates well with targeted applications such as parallel reaction monitoring (PRM). Acknowledgement: "This work was supported by the Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry (RRID:SCR_017801) utilizing the Thermo QE-HFX mass spectrometer (RRID:SCR_018703).
Thermo Orbitrap Fusion nanoLC/MS
The Thermo Orbitrap Fusion Tribrid mass spectrometer and Waters M-Class Acquity nanoUPLC is a versatile platform, featuring sequential acquisition capabilities and multiple fragmentation types, that enable complex, in depth proteomic experiments. Acknowledgement: "This work was supported by the Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry (RRID:SCR_017801) utilizing the Thermo Orbitrap Fusion nanoLC/MS system (RRID:SCR_018702).
Shimadzu 8030 LC/MS
The Shimadzu 8030 triple quadrupole mass spectrometer is a workhorse for targeted quantitation applications. Acknowledgement: "This work was supported by the Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry (RRID:SCR_017801) utilizing the Shimadzu 8030 LC/MS mass spectrometer (RRID:SCR_018699).
Waters Xevo TQ-XS LC/MS
The Xevo TQ-XS is our newest triple quad, providing cutting-edge targeted quantitation capabilities with both analytical and microflow UPLC separations. The Xevo was acquired in 2019 with federal funding that must be acknowledged: "This work utilized the Xevo TQ-XS mass spectrometer system (RRID:SCR_018705) that was purchased with funding from National Institutes of Health Shared Instrumentation grant S10OD026962, and was supported by Stanford University Mass Spectrometry (RRID:SCR_017801)."
Waters Quattro Premier LC/MS
The Micromass (now Waters) Quattro Premier triple quadrupole operates with an Agilent 1100 HPLC and specializes in quantitative work and selective of targeted analytes including metabolites and other small molecules, and peptides from complex matrices such as serum, tissue, cell culture, and other biological sources. Acknowledgement: "This work was supported by the Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry (RRID:SCR_017801) utilizing the Waters Quattro Premier LC/MS system (RRID:SCR_018705).
Waters SQD2 LC/MS
The Waters SQD2 is a single quadrupole instrument equipped with reversed phase LC/MS columns and electrospray ionization. The SQD2 runs under Remote Analyzer open access software, and trained open access users select from a menu of standard methods to analyze their samples. Acknowledgement: "This work was supported by the Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry (RRID:SCR_017801) utilizing the Waters SQD2 LC/MS system (RRID:SCR_022217).
Bruker Microflex MALDI-TOF
The Microflex LRF is specialized for protein, peptide, and polymer analysis. This MALDI-TOF instrument can be operated in a linear mode for high mass accuracy characterization, or in a reflector mode to achieve enhanced resolution. Acknowledgement: "This work was supported by the Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry (RRID:SCR_017801) utilizing the Bruker Microflex MALDI TOF mass spectrometer (RRID:SCR_018696).
Agilent 7890/5975 GC/MS
The Agilent GC/MS is a single quadrupole instrument with electron ionization (EI) that generates "fingerprint" fragment spectra for volatile compounds. The GC/MS runs under ChemStation open access software, and trained open access users select from a menu of standard methods to analyze their samples.