2020 SUMS-RAS Breakouts
MASS SPEC: THE NEXT GENERATION
Wed-Thurs October 28-29, 2020
9 am-2 pm Pacific Time, online
Wednesday October 28, 2020
1:15 - 2:00 pm Pacific Time
Breakouts are held in Zoom meeting rooms unless otherwise stated
Open discussion on next generation technologies and modernizing your lab
Come join us for an interactive session including a short demonstration on automatic sample preparation with the Andrew Alliance robot, discussions around mass spec imaging with some cool examples, and applications of cyclic IMS to answer everyday research questions. Whether you are just curious about what we have to say, or want to come with specific questions, we are here to help!
Collision Induced Unfolding: A New Paradigm in Rapid Protein Stability Measurements and Deep Structural Characterization
Collision induced unfolding (CIU) uses Agilent 6560 ion mobility-mass spectrometry (IM-MS) to measure the stability and unfolding pathways of gas-phase protein complexes, without the need for covalent labels or tagging and consuming much less sample than almost any other label-free technology to differentiate therapeutic antibodies, improve the throughput, and enhance the information content. The combination of CIU and electron capture dissociation (ECD) on an ion mobility mass spectrometer CIU-IMS(CCS)-ECD improves structural characterization of a larger variety of biological molecules, such as peptides, oligonucleotides, oligosaccharides, metabolites, and lipids. Fragmentation patterns of both positive and negative ions are investigated and should provide insights for a deeper understanding of these processes.
Both Professor Brandon Ruotolo and Professor Kristina HÃ¥kansson will be at Agilent Breakout session to answer questions.
ProAlanase: A New Proline- and Alanine-Specific Protease for Proteomics
Presented by Chris Hosfield, Sr. Research Scientist 2, Promega Corporation
Proteases beyond trypsin are important in proteomic analyses since they can be used to increase sequence coverage and identify post-translational modifications. Like trypsin, the most commonly used site-specific alternative proteases perform cleavage at charged residues. There is a need for site-specific proteases that cleave at unique sites in the proteome. Here we report the characterization of ProAlanase; a protease which preferentially cleaves proteins on the C-terminal side of proline, and to a lesser extent, alanine residues. ProAlanase appears to have broad potential for proteomics applications including de novo sequencing, histone PTM analysis, phosphoprofiling, ancient bone protein identification and disulfide bond mapping.
Bringing the Power of MALDI Mass Spec to Every Lab Bench
Presented by Stephen Kurzyniec, Pharma/BioPharma Business Development Specialist
https://www.ssi.shimadzu.com/products/maldi-tof-mass-spectrometry/index....
Thursday October 29, 2020
12:45 - 1:30 pm Pacific Time
Breakouts are held in Zoom meeting rooms
- spectrum filtering
- glycan wildcard search
- user-customizable scoring and peak labeling
These new features enable search for unusual or unnatural PTMs, including chemical biology handles.
Real-time database search is a reality, ions in and peptide sequences out! Join us to find out more
Presented by Chris Adams, Global Business and Product Manager Integrated Proteomics
The timsTOF Pro allows the analysis of hundreds of samples with minimal sample load (<200 ng) at sequencing speeds exceeding 100 Hz. All of this with uncompromised proteomic depth. This has changed the way proteomics is done, but it also unleashes a new constraint - data analysis. Modern researchers must process hundreds of samples of information dense content with the timsTOF Pro. For most pipelines this creates a bottleneck at the data analysis step. Bruker is innovating this step by introducing real-time database search capabilities called PaSER (Parallel database Search Engine in Real-time) which removes the data analysis hurdle. Using PaSER, as soon as a LC-MS run is completed, results are in hand or Run & Done.
https://www.youtube.com/watch?v=Bywxk9pml_0&feature=youtu.be
Come discuss your sample preparation needs and hear about the mass spectrometry workflows we have developed
Do you have any sample preparation issues, questions, or perhaps you'd like some custom micro-particles? Please join us for a relaxed and informal session where we'll provide a short company overview and allow you the opportunity to discuss aspects of proteomics sample preparation relevant to your research.
Profiling the Lipidome: Quantification of over 2000 lipids in a single injection!
The complexity of the lipidome is very high with a huge diversity in lipid molecular species making comprehensive quantitative profiling challenging. In this assay, MRMs to quantitate over 2000 lipid molecular species were combined into a single injection on the SCIEX 7500 System. The target list of lipids is comprehensive, covering 21 lipid classes, and MRMs were selected to cover lipids containing fatty acids with 14 to 26 carbons and 0 to 6 double bonds. The method is customizable, so new lipid categories, classes and molecular species can be added to the MRMs list and is matrix agnostic.